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sam pooled sgrna library  (Addgene inc)


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    Addgene inc sam pooled sgrna library
    Sam Pooled Sgrna Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+crispr+activation+library+sam-3+system/Human+CRISPR+Activation+Library+(SAM+-+3+plasmid+system)+(Pooled+Library+%231000000057%2C+%231000000074)/pmc12339858-5-45-49
    Average 93 stars, based on 27 article reviews
    sam pooled sgrna library - by Bioz Stars, 2026-09
    93/100 stars

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    ( A ) FGFR2, BACH2 and MET were the only commonly enriched hits identified by CRISPRa screens conducted in both cell lines (CHLA20 and SH-SY5Y) with a beta score>1; p <0.01. ( B ) Significantly enriched genes (beta score>1, p<0.01, permutation-based non-parametric analysis (PBNPA) ) from CRISPRa screens conducted in CHLA20 cells. The gRNAs are ranked by fold-change vs difference in log-normalized read counts between DMSO and lorlatinib-treated CHLA20 cells. Two gRNAs per gene are shown and the <t>gRNA</t> to FGFR2 is highlighted in blue. ( C, D ) FGFR2 and its regulated pathways are enriched in CHLA20 NB cells following 14 days of lorlatinib treatment compared to DMSO treated cells: GSEA with GO gene sets in MSigDB in C and Reactome in D. ( E ) FGFR2 expression levels in CHLA20-dCas9 cells transduced with one of two independent FGFR2 gRNAs (FGFR2-1 and FGFR2-2) compared to non-targeting (NT) gRNA transduced cells. ( F ) Viability (cell-titer blue (CTB)) of CHLA20-Cas9 cells transduced with NT gRNA or two different FGFR2 gRNAs following treatment with the indicated doses of lorlatinib for 72 h. (G) EC 50 values calculated from (F) are shown as means ±SEM from three biological replicates. Significance was determined using a two-tailed Student’s t-test, **p=0.0066.
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    ( A ) FGFR2, BACH2 and MET were the only commonly enriched hits identified by CRISPRa screens conducted in both cell lines (CHLA20 and SH-SY5Y) with a beta score>1; p <0.01. ( B ) Significantly enriched genes (beta score>1, p<0.01, permutation-based non-parametric analysis (PBNPA) ) from CRISPRa screens conducted in CHLA20 cells. The gRNAs are ranked by fold-change vs difference in log-normalized read counts between DMSO and lorlatinib-treated CHLA20 cells. Two gRNAs per gene are shown and the gRNA to FGFR2 is highlighted in blue. ( C, D ) FGFR2 and its regulated pathways are enriched in CHLA20 NB cells following 14 days of lorlatinib treatment compared to DMSO treated cells: GSEA with GO gene sets in MSigDB in C and Reactome in D. ( E ) FGFR2 expression levels in CHLA20-dCas9 cells transduced with one of two independent FGFR2 gRNAs (FGFR2-1 and FGFR2-2) compared to non-targeting (NT) gRNA transduced cells. ( F ) Viability (cell-titer blue (CTB)) of CHLA20-Cas9 cells transduced with NT gRNA or two different FGFR2 gRNAs following treatment with the indicated doses of lorlatinib for 72 h. (G) EC 50 values calculated from (F) are shown as means ±SEM from three biological replicates. Significance was determined using a two-tailed Student’s t-test, **p=0.0066.

    Journal: bioRxiv

    Article Title: FGFR2 promotes resistance to ALK tyrosine kinase inhibitors and its inhibition acts synergistically with lorlatinib in the treatment of ALK-expressing neuroblastoma

    doi: 10.1101/2024.09.05.611416

    Figure Lengend Snippet: ( A ) FGFR2, BACH2 and MET were the only commonly enriched hits identified by CRISPRa screens conducted in both cell lines (CHLA20 and SH-SY5Y) with a beta score>1; p <0.01. ( B ) Significantly enriched genes (beta score>1, p<0.01, permutation-based non-parametric analysis (PBNPA) ) from CRISPRa screens conducted in CHLA20 cells. The gRNAs are ranked by fold-change vs difference in log-normalized read counts between DMSO and lorlatinib-treated CHLA20 cells. Two gRNAs per gene are shown and the gRNA to FGFR2 is highlighted in blue. ( C, D ) FGFR2 and its regulated pathways are enriched in CHLA20 NB cells following 14 days of lorlatinib treatment compared to DMSO treated cells: GSEA with GO gene sets in MSigDB in C and Reactome in D. ( E ) FGFR2 expression levels in CHLA20-dCas9 cells transduced with one of two independent FGFR2 gRNAs (FGFR2-1 and FGFR2-2) compared to non-targeting (NT) gRNA transduced cells. ( F ) Viability (cell-titer blue (CTB)) of CHLA20-Cas9 cells transduced with NT gRNA or two different FGFR2 gRNAs following treatment with the indicated doses of lorlatinib for 72 h. (G) EC 50 values calculated from (F) are shown as means ±SEM from three biological replicates. Significance was determined using a two-tailed Student’s t-test, **p=0.0066.

    Article Snippet: The puromycin-resistant pooled gRNA library v1 (Addgene, Cat#1000000057) was packaged into lentivirus in 15 cm plates, as described above.

    Techniques: Expressing, Transduction, Two Tailed Test